The Effect of Duration of Wound Skin Tissue on MDA Plasma Level and Micro Vessel Density (CD34 Expression) in White Rats (Rattus novergicus)

Aims: To determine the effect of wound duration on plasma MDA levels and Micro Vessel Density (CD34 Expression) in white rats (Rattus novergicus).

Study Design: Experimental post-test only control group design.

Place and Duration of Study: Animal care tried PAU UGM Yogyakarta, while immunohistochemical observations were conducted by experts in that field at the Anatomical Pathology Faculty of Medicine, University of Sebelas Maret Surakarta, Indonesia, between March and June 2020.

Methodology: We included male white rats (Rattus Norvegicus) which consists of 18 samples divided into 3 groups, namely the group with 5 days of wound duration (K1), the group with 10 days of injury (K2), and the group with a wound length of 15 days (K3). The wound is made a square incision with a length of 2x2 cm. The parameters assessed were plasma MDA levels and Micro Vessel Density (CD34 Expression). Statistical analysis of plasma MDA levels and Micro Vessel Density (CD34 Expression) using OneWay ANOVA followed by Tukey HSD.

Results: The OneWay ANOVA test results showed significant values in plasma MDA levels (P=0.000) and CD34 Expression (P=0.001). The Tukey HSD test results showed a significant value (P <0.05) in the K1 with K2, K1 and K3 groups and K2 with K3 groups on the parameters of MDA plasma. The Tukey HSD test results showed a significant value (P <0.05) in the K1 with K2 and K1 with K3groups but not significant (P=0.572) in the K2 with K3 groups on the parameters of CD34 expressions.

Conclusion: Longest duration of the wound has an effect on decreasing levels of plasma MDA levels and Micro Vessel Density (CD34 Expression) in the wound healing process.